Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 1

Creative Commons Licence
Imaging technique: 
Preparation technique: 
Micro-computed tomography

Lumbrineris latreilli, a) false-colour volume rendering of the anterior region, dorso-lateral view, specimen partly covered by a thin film of ethanol; b) surface model of the vascular system (yellow) and brain (purple) superimposed on volume rendering of the worm, dorso-anterior view; c) false-colour, semi-transparent volume rendering of the anterior region, dorsal view; d) volume rendering of virtually dissected anterior region, lateral view.

Sample preparation:
Animal was fixed in formalin and preserved in 70% Ethanol. For scanning it was removed from the preservation medium and placed into a polypropylene pipette tip. Remaining liquids were removed with paper tissue and the tip was sealed to prevent drying out.

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images:  1140
Rotation step: 0.25°
Exposure time per image: 1925 ms
Total scanning time: 3:08h
Resolution (µm/pixel):1.919

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)
Software used for creation of surface model: Amira v. 5.2

Sarah Faulwetter