Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 8

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Imaging technique: 
Preparation technique: 
Micro-computed tomography

Eunice roussaeii, surface model of mid-body parapodium. Limbate chaetae, pectinate chaetae and notochaetae are not shown. Compound chaetae have been included as simplified, remodelled version and do not depict true shapes. The interactive 3D-mode can be activated by clicking on the image, allowing to rotate, move and magnify the model, to isolate elements and to change the light settings. Use mouse buttons to rotate and zoom, CTRL+ left mouse button to translocate. Use the 3D toolbar for further interaction.  

Possible incompatibilities:  Interactive 3D models should theoretically be supported by most Acrobat products (Reader, Professional) from Version 8 onwards. However, on some operating systems or when viewed from within specific browsers (e.g. Chrome), incompatibilities might occur. In this case, please try to download the PDF and open it locally on your computer with an Acrobat product. If still you are unable to view the model, you can download it in various formats below and open it with a 3D viewer of your choice.

Sample preparation:
Specimen was fixed and preserved in 96% ethanol. Prior to scanning, a parapod was dissected and it was desiccated with Hexamethyldisilazane. The dried sample was fixed in a piece of styrofoam for scanning. 

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images: 1440
Rotation step: 0.25°
Exposure time per image: 1155 ms
Total scanning time: 2:19h
Resolution (µm/pixel):2.878

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for creating the surface model:   Amira v. 5.2 (Visage Imaging, Berlin, Germany)

Sarah Faulwetter