Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 7
Eunice roussaei, mid-body parapodium, a) cross section through parapodial base; b) semi-transparent volume rendering; c) opaque volume rendering; d) volume rendering of chaetae.
Specimen was fixed and preserved in 96% ethanol. Prior to scanning, a parapod was dissected and it was desiccated with Hexamethyldisilazane. The dried sample was fixed in a piece of styrofoam for scanning.
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Nr of projection images: 1440
Rotation step: 0.25°
Exposure time per image: 1155 ms
Total scanning time: 2:19h
Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for creating the surface model: Amira v. 5.2 (Visage Imaging, Berlin, Germany)