Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 9
Alitta succinea, a) normal view and b) stereo view of volume-rendering of anterior region, lateral view (3D effect is revealed when viewed with red-cyan glasses); c) coronal cross section of anterior region depicting areas of insufficient tissue staining with PTA. Black areas in pharyngeal region remained unstained and information is lost; d) dorsal view and e) posterior-lateral view of false-colour volume rendering of virtually dissected anterior region; f) volume rendering of anterior region, dorsal view.
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 20 days in 0.3% phosphotungstic acid (PTA) in 70% ethanol, the solution was renewed every 5 days. For scanning, it was placed into a polypropylene pipette tip in 96% Ethanol.
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Nr of projection images: 900
Rotation step: 0.4°
Exposure time per image: 190 ms
Total scanning time: 2x 0:17h (multi-part scan, subscans automatically combined)
Camera pixel binning: 2x2
Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)