Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 10
Phyllodoce lineata, a) false-colour volume rendering of anterior region, dorso-lateral view; b) false-colour volume rendering of virtually dissected distal end of partly everted pharynx, focusing on terminal pharyngeal papillae, anterior view; c) transaxial cross section at eye level; d) volume rendering of virtually dissected anterior region showing the brain, dorso-posterior view; e) same, in stereo view (3D effect is revealed when viewed with red-cyan glasses).
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 36hrs in 0.3% phosphotungstic acid (PTA) in 70% ethanol. For scanning, it was placed into a polypropylene pipette tip in 96% ethanol.
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Nr of projection images: 1285
Rotation step: 0.28°
Exposure time per image: 1165 ms
Total scanning time: 2:04h
Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)