Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 10

Creative Commons Licence
Imaging technique: 
Preparation technique: 
Micro-computed tomography

Phyllodoce lineata, a) false-colour volume rendering of anterior region, dorso-lateral view; b) false-colour volume rendering of virtually dissected distal end of partly everted pharynx, focusing on terminal pharyngeal papillae, anterior view; c) transaxial cross section at eye level; d) volume rendering of virtually dissected anterior region showing the brain, dorso-posterior view; e) same, in stereo view (3D effect is revealed when viewed with red-cyan glasses).

Sample preparation:
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 36hrs in 0.3% phosphotungstic acid (PTA) in 70% ethanol. For scanning, it was placed into a polypropylene pipette tip in 96% ethanol.

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images:  1285
Rotation step: 0.28°
Exposure time per image: 1165 ms
Total scanning time: 2:04h
Resolution (µm/pixel):1.096

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)

Sarah Faulwetter