Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 11

Licence: 
Creative Commons Licence
Imaging technique: 
Other
Preparation technique: 
Micro-computed tomography
Description: 

a) Phyllodoce lineata, volume rendering of a) anterior end, virtually dissected behind prostomial region, posterior view; b) Phyllodoce sp., volume rendering of virtually dissected pharynx, dorsal view; c) Phyllodoce sp., virtual dissection of distal pharyngeal subdivision with large papillae, anterior view; d) Phyllodoce sp., virtual dissection of proximal pharyngeal subdivision with small papillae, antero-lateral view.

Sample preparation (Phyllodoce lineata):
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 36hrs in 0.3% phosphotungstic acid (PTA) in 70% ethanol. For scanning, it was placed into a polypropylene  pipette tip in 96% ethanol.

Scanning parameters (Phyllodoce lineata):
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images:  1285
Rotation step: 0.28°
Exposure time per image: 1165 ms
Total scanning time: 2:04h
Resolution (µm/pixel):1.096

Sample preparation (Phyllodoce sp):
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 36hrs in 0.3% phosphotungstic acid (PTA) in 70% ethanol. For scanning, it was placed into a polypropylene pipette tip in 96% ethanol.

Scanning parameters (Phyllodoce sp):
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images:  1285
Rotation step: 0.28°
Exposure time per image: 1336 ms
Total scanning time: 2:05h
Resolution (µm/pixel):1.645

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)

Creator: 
Sarah Faulwetter