Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 12

Creative Commons Licence
Imaging technique: 
Preparation technique: 
Micro-computed tomography

Syllis gracilis, false-colour volume rendering of a) anterior region, anterior view; b) anterior region virtually dissected at pharyngeal level, posterior view; c) virtually dissected anterior region showing the brain, anterior view; d) virtually dissected region in front of proventricle, dorsal view; e) mid-body parapodia, posterior-lateral view.

Sample preparation:
Animal was fixed and preserved in 96% ethanol. Prior to scanning, it was stained for 3 days in a 1% iodine solution in 96% ethanol. For scanning, it was placed into a polypropylene pipette tip in 96% Ethanol.

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images: 1440
Rotation step: 0.25°
Exposure time per image: 1165 ms
Total scanning time: 3x 1:52h (multi-part scan, subscans automatically combined)
Resolution (µm/pixel): 1.439

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: Drishti

Sarah Faulwetter