Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 14

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Imaging technique: 
Preparation technique: 
Micro-computed tomography

Syllis gracilis, false-colour volume rendering of virtually dissected proventricle.

Sample preparation:
Animal was fixed and preserved in 96% ethanol. Prior to scanning, it was stained for 3 days in a 1% iodine solution in 96% ethanol. For scanning, it was placed into a polypropylene pipette tip in 96% Ethanol.

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images: 1440
Rotation step: 0.25°
Exposure time per image: 1165 ms
Total scanning time: 3x 1:52h (multi-part scan, subscans automatically combined)
Resolution (µm/pixel): 1.439

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: Drishti

Sarah Faulwetter