Micro-computed tomography: Introducing new dimensions to taxonomy. Figure 15

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Imaging technique: 
Preparation technique: 
Micro-computed tomography

Hermodice carunculata, false-colour volume rendering of a) anterior region, antero-dorsal view; b) virtually dissected anterior end showing pharyngeal structures, lateral view; c) virtually dissected anterior end, lateral view; d) virtually dissected anterior end at level of mouth opening, anterior view.

Sample preparation:
Animal was fixed and preserved in 96% ethanol. Prior to scanning, it was desiccated with Hexamethyldisilazane. The dried animal was fixed in a piece of styrofoam for scanning.  

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images: 1200
Rotation step: 0.3°
Exposure time per image: 155 ms
Total scanning time: 0:32h
Resolution (µm/pixel):8.922

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)
Software used for volume rendering: CTVox (SkyScan)

Sarah Faulwetter