Alitta_eyes.png

Licence: 
Creative Commons Licence
Imaging technique: 
micro-CT
Description: 

Sample preparation:
Animal was fixed in formalin and preserved in 70% ethanol. Prior to scanning, it was stained for 20 days in 0.3% phosphotungstic acid (PTA) in 70% ethanol, the solution was renewed every 5 days. For scanning, it was placed into a polypropylene pipette tip in 96% Ethanol.

Scanning parameters:
Scanner: SkyScan 1172
Voltage/ Current: 60kV/ 167µA
Filter: None
Nr of projection images: 900
Rotation step: 0.4°
Exposure time per image: 190 ms
Total scanning time: 2x 0:17h (multi-part scan, subscans automatically combined)
Resolution (µm/pixel):3.425
Camera pixel binning: 2x2

Reconstruction & Visualisation:
Software used for reconstruction: NRecon (SkyScan)